from Bacillus stearothermophilus
|Specific activity||:||more than 5,000 U/mg protein|
|Contaminants||:||(as PTA activity = 100%)|
|Molecular weight||:||ca. 70,000|
|Subunit molecular weight||:||ca. 35,000|
|pH stability||:||7.0 - 11.0||(Fig.2)|
|Thermal stability||:||No detectable decrease in activity up to 50°C.||(Fig.3, 4)|
|Michaelis constants||:||(87mM Tris-HCl buffer, pH 7.5, at 30°C)|
stable at -20°C for at least one year
The enzyme is useful for determination of CoA or acetate.
The change in absorbance is measured at 233 nm according to the following reaction.
One unit of activity is defined as the amount of PTA that forms 1 µmol of acetyl-CoA per minute at 30°C.
Preparation of Enzyme Solution
Dissolve the lyophilized enzyme with distilled water and dilute to 5 to 20 U/mL with 50mM Tris-HCl buffer, pH 8.0.
|Solution I||26.0mL||Solution III||1.0mL|
|Solution II||2.0mL||Solution IV||1.0mL|