|Specific activity||:||more than 1,200 U/mg protein|
|Contaminants||:||(as MDH activity = 100%)|
|Molecular weight||:||ca. 72,000|
|Subunit molecular weight||:||ca. 36,000|
|pH stability||:||5.5 - 11.0||(Fig.2)|
|Thermal stability||:||No detectable decrease in activity
up to 50°C.
|Michaelis constants||:||(90mM Tris-HCl buffer, pH 9.0, at 30°C)|
stable at -20°C for at least six months
This enzyme is useful for enzymatic determination of L- malate and of glutamate oxaloacetate transaminase in clinical analysis.
The change in absorbance is measured at 340 nm according to the following reaction.
One unit of activity is defined as the amount of MDH that forms 1µmol of NAD+ per minute at 30°C.
Preparation of Enzyme Solution
Dissolve the lyophilized enzyme with distilled water and dilute to 3 to 5 U/mL with 100mM Tris-HCl buffer, pH 9.0.
|Solution I||13.50mL||Solution III||0.57mL|